2023
Design, construction, and functional characterization of a tRNA neochromosome in yeast Cover Journal Article
In: Cell, vol. 186, iss. 24, pp. 5237–5253, 2023.
Establishing chromosomal design-build-test-learn through a synthetic chromosome and its combinatorial reconfiguration Journal Article
In: Cell Genomics, vol. 3, iss. 11, no. 100435, 2023.
Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics Journal Article
In: Cell Genomics, vol. 3, iss. 11, no. 100418, 2023.
Parallel laboratory evolution and rational debugging reveal genomic plasticity to S. cerevisiae synthetic chromosome XIV defects Journal Article
In: Cell Genomics, vol. 3, iss. 11, no. 100379, 2023.
Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast Cover Journal Article
In: Cell Genomics, vol. 3, iss. 11, no. 100364, 2023.
A spotlight on global collaboration in the Sc2.0 yeast consortium Journal Article
In: Cell Genomics, vol. 3, iss. 11, no. 100441, 2023.
Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions Journal Article
In: Cell, 2023.
Cellular Surveillance: DNA-Based Recording to Monitor and Memorize Biological Events Journal Article
In: GEN Biotechnology, vol. 2, no. 3, 2023.
Safety by design: Biosafety and biosecurity in the age of synthetic genomics Journal Article
In: iScience, vol. 26, iss. 3, pp. 106165, 2023.
2022
Engineering stringent genetic biocontainment of yeast with a protein stability switch Journal Article
In: bioRxiv, 2022.
Reconstruct a eukaryotic chromosome arm by de novo design and synthesis Journal Article
In: bioRxiv, 2022.
Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast Journal Article
In: bioRxiv, 2022.
Laboratory evolution and polyploid SCRaMbLE reveal genomic plasticity to synthetic chromosome defects and rearrangements Journal Article
In: bioRxiv, 2022.
Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand Journal Article
In: bioRxiv, 2022.
Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions Journal Article
In: bioRxiv, 2022.
New Opportunities for Genetic Code Expansion in Synthetic Yeast Journal Article
In: Current Opinion in Biotechnology, vol. 75, no. 102691, 2022.
Automation and Expansion of EMMA Assembly for Fast-Tracking Mammalian System Engineering Journal Article
In: ACS Synthetic Biology, vol. 11, no. 2, pp. 587–595, 2022.
EMMA-CAD: Design Automation for Synthetic Mammalian Constructs Journal Article
In: ACS Synthetic Biology, vol. 11, no. 2, pp. 579–586, 2022.
2021
SCRaMbLE: A Study of Its Robustness and Challenges through Enhancement of Hygromycin B Resistance in a Semi-Synthetic Yeast Journal Article
In: Bioengineering (Basel), vol. 8, no. 3, pp. 42, 2021.
Compacting a synthetic yeast chromosome arm Journal Article
In: Genome Biology, vol. 22, no. 5, 2021.
2020
SpEDIT: A fast and efficient CRISPR/Cas9 method for fission yeast Journal Article
In: Wellcome open research, vol. 5, pp. 274, 2020.
Sc3.0: revamping and minimizing the yeast genome Journal Article
In: Genome Biology, vol. 21, no. 205, 2020.
Probing eukaryotic genome functions with synthetic chromosomes Journal Article
In: Experimental Cell Research, vol. 390, no. 1, pp. 111936, 2020.
The COVID-19 XPRIZE and the need for scalable, fast, and widespread testing Journal Article
In: Nature Biotechnology, vol. 38, pp. 1021–1024, 2020.
SCRaMbLE-in: A Fast and Efficient Method to Diversify and Improve the Yields of Heterologous Pathways in Synthetic Yeast Book Section
In: DNA Cloning and Assembly, vol. 2205, pp. 305–327, 2020.
Leveraging the Hermes Transposon to Accelerate the Development of Nonconventional Yeast-based Microbial Cell Factories Journal Article
In: ACS Synthetic Biology, vol. 9, no. 7, pp. 1736–1752, 2020.
2019
Multiplex Genome Engineering for Optimizing Bioproduction in Saccharomyces cerevisiae Journal Article
In: Biochemistry, vol. 58, no. 11, pp. 1492–1500, 2019.
Building a global alliance of biofoundries Journal Article
In: Nature Communications, vol. 10, pp. 2040, 2019.
EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors Journal Article
In: Methods in Enzymology, vol. 617, pp. 463–493, 2019.
Improving Chromosome Synthesis with a Semiquantitative Phenotypic Assay and Refined Assembly Strategy Journal Article
In: ACS Synthetic Biology, vol. 8, no. 10, pp. 2203–2211, 2019.
2018
Hydrogen Peroxide-Based Fluorometric Assay for Real-Time Monitoring of SAM-Dependent Methyltransferases Journal Article
In: Frontiers in Bioengineering and Biotechnology, vol. 6, pp. 146, 2018.
Synthetic genomics: a new venture to dissect genome fundamentals and engineer new functions Journal Article
In: Current Opinion in Chemical Biology, vol. 46, pp. 56–62, 2018.
YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast Journal Article
In: Methods in Enzymology, vol. 608, pp. 277–306, 2018.
Artificial Protein Scaffold System (AProSS): An efficient method to optimize exogenous metabolic pathways in Saccharomyces cerevisiae Journal Article
In: Metabolic Engineering, vol. 49, pp. 13–20, 2018.
Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods Journal Article
In: Nature Communications, vol. 9, pp. 1936, 2018.
Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES Journal Article
In: Nature Communications, vol. 9, pp. 1930, 2018.
Whole genome engineering by synthesis Journal Article
In: Science China Life Sciences, vol. 61, no. 12, pp. 1515–1527, 2018.
2017
Low escape-rate genome safeguards with minimal molecular perturbation of Saccharomyces cerevisiae Journal Article
In: Proceedings of the National Academy of Sciences, vol. 114, pp. 1470– 1479, 2017.
Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome Journal Article
In: Cold Spring Harbor Protocols, vol. 2017, no. 3, 2017.
LeafLIMS: A Flexible Laboratory Information Management System with a Synthetic Biology Focus Journal Article
In: ACS Synthetic Biology, vol. 6, no. 12, pp. 2273–2280, 2017.
Orthogonal Ribosome Biofirewall Journal Article
In: ACS Synthetic Biology, vol. 6, no. 11, pp. 2108–2117, 2017.
Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways Journal Article
In: Methods in Molecular Biology, vol. 1642, pp. 285–302, 2017.
EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors Journal Article
In: ACS Synthetic Biology, vol. 6, no. 7, pp. 1380–1392, 2017.
3D organization of synthetic and scrambled chromosomes Journal Article
In: Science, vol. 355, no. 6329, 2017.
Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond Journal Article
In: Science, vol. 355, no. 6329, 2017.
Design of a synthetic yeast genome Journal Article
In: Science, vol. 355, no. 6329, pp. 1040–1044, 2017.
Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome Cover Journal Article
In: Science, vol. 355, no. 6329, 2017.
Bug mapping and fitness testing of chemically synthesized chromosome X Journal Article
In: Science, vol. 353, no. 6329, 2017.
“Perfect” designer chromosome V and behavior of a ring derivative Journal Article
In: Science, vol. 355, no. 6329, 2017.