Publications

@article{swjbw23:cell,

title = {Design, construction, and functional characterization of a tRNA neochromosome in yeast},

author = {D. Schindler and R. S. K. Walker and S. Jiang and A. N. Brooks and Y. Wang and C. A. Müller and C. Cockram and Y. Luo and A. Garcia and D. Schraivogel and J. Mozziconacci and N. Pena and M. Assari and M. d. C. S. Olmos and Y. Zhao and A. Ballerini and B. A. Blout and J. Cai and L. Ogunlana and W. Liu and K. Jönsson and D. Abramczyk and E. Garcia-Ruiz and T. W. Turowski and R. Swidah and T. Ellis and T. Pan and F. Antequera and Y. Shen and C. A. Nieduszynski and R. Koszul and J. Dai and L. M. Steinmetz and J. D. Boeke and Y. Cai},

doi = {doi.org/10.1016/j.cell.2023.10.015},

year  = {2023},

date = {2023-11-24},

urldate = {2023-11-24},

journal = {Cell},

volume = {186},

issue = {24},

pages = {5237--5253},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{fksjl23:cell_genomics,

title = {Establishing chromosomal design-build-test-learn through a synthetic chromosome and its combinatorial reconfiguration},

author = {J. L. Foo and S. Kitano and A. V. Susanto and Z. Jin and Y. Lin and Z. Luo and L. Huang and Z. Liang and L. A. Mitchell and K. Yang and A. Wong and Y. Cai and J. Cai and G. Stracquadanio and J. S. Bader and J. D. Boeke and J. Dai and M. W. Chang},

doi = {10.1016/j.xgen.2023.100435},

year  = {2023},

date = {2023-11-08},

urldate = {2023-11-08},

journal = {Cell Genomics},

volume = {3},

number = {100435},

issue = {11},

key = {fksjl23:cell_genomics},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{bldjs23:cell_genomics,

title = {Synthetic yeast chromosome XI design provides a testbed for the study of extrachromosomal circular DNA dynamics},

author = {B. A. Blout and X. Lu and M. R. M. Driessen and D. Jovicevic M. I. Sanchez and K. Ciurkot and Y. Zhao and S. Lauer and R. M. McKiernan and G.-O. F. Gowers and F. Sweeney and V. Fanfani and E. Lobzaev and K. Palacios-Flores and R. S. K. Walker and A. Hesketh and J. Cai and S. G. Oliver and Y. Cai and G. Stracquadanio and L. A. Mitchell and J. S. Bader and J. D. Boeke and T. Ellis},

doi = {doi.org/10.1016/j.xgen.2023.100418},

year  = {2023},

date = {2023-11-08},

urldate = {2023-11-08},

journal = {Cell Genomics},

volume = {3},

number = {100418},

issue = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{wkxwl23:cell_genomics,

title = {Parallel laboratory evolution and rational debugging reveal genomic plasticity to S. cerevisiae synthetic chromosome XIV defects},

author = {T. C. Williams and H. Kroukamp and X. Xu and E. L. I. Wightman and B. Llorente and A. R. Borneman and A. C. Carpenter and N. Van Wyk and F. Meier and T. R. V. Collier and M. I. Espinosa and E. L. Daniel and R. S. K. Walker and Y. Cai and H. K. M. Nevalainen and N. C. Curach and I. W. Deveson and T. R. Mercer and D. L. Johnson and L. A. Mitchenll and J. S. Bader and G. Stracquadanio and J. D. Boeke and H. D. Goold and I. S. Pretorius and I. T. Paulsen},

doi = {doi.org/10.1016/j.xgen.2023.100379},

year  = {2023},

date = {2023-11-08},

journal = {Cell Genomics},

volume = {3},

number = {100379},

issue = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{sgwwz23:cell_genomics,

title = {Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast},

author = {Y. Shen and F. Gao and Y. Wang and Y. Wang and J. Zheng and J. Gong and J. Zhang and Z. Luo and D. Schindler and Y. Deng and W. Ding and T. Lin and R. Swidah and H. Zhao and S. Jiang and C. Zeng and S. Chen and T. Chen and Y. Wang and Y. Luo and L. Mitchell and J. S. Bader and G. Zhang and X. Shen J. Wang and X. Fu and J. Dai and J. D. Boeke and H. Yang and X. Xu and Y. Cai},

doi = {doi.org/10.1016/j.xgen.2023.100364},

year  = {2023},

date = {2023-11-08},

urldate = {2023-11-08},

journal = {Cell Genomics},

volume = {3},

number = {100364},

issue = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dypcs23:cell_genomics,

title = {A spotlight on global collaboration in the Sc2.0 yeast consortium},

author = {J. Dai and H. Yang and I. S. Pretorius and Y. Cai and Y. Shen and M. Chang and Y. Yuan},

doi = {doi.org/10.1016/j.xgen.2023.100441},

year  = {2023},

date = {2023-11-08},

journal = {Cell Genomics},

volume = {3},

number = {100441},

issue = {11},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zchly23:cell_genomics,

title = {Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions},

author = {Y. Zhao and C. Coelho and A. L. Hughes and L. Lazar-Stefanita and S. Yang and A. N. Brooks and R. S. K. Walker and W. Zhang and S. Lauer and C. Hernandez and J. Cai and L. A. Mitchell and N. Agmon and Y. Shen and J. Sall and V. Fanfani and A. Jalan and J. Rivera and F.-X. Liang and J. S. Bader and G. Stracquadanio and L. M. Steinmetz and Y. Cai and J. D. Boeke},

doi = {doi.org/10.1016/j.cell.2023.09.025},

year  = {2023},

date = {2023-11-08},

journal = {Cell},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{doc23:gen_biotechnology,

title = {Cellular Surveillance: DNA-Based Recording to Monitor and Memorize Biological Events},

author = {G. David and R. T. O'Keefe and Y. Cai},

doi = {doi:10.1089/genbio.2023.0018},

year  = {2023},

date = {2023-06-19},

urldate = {2023-06-19},

journal = {GEN Biotechnology},

volume = {2},

number = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{hdddk23:iscience,

title = {Safety by design: Biosafety and biosecurity in the age of synthetic genomics},

author = {S. A. Hoffmann and J. Diggans and D. Densmore and J. Dai and T. Knight and E. Leproust and J. D. Boeke and N. Wheeler and Y. Cai},

doi = {10.1016/j.isci.2023.106165},

year  = {2023},

date = {2023-03-17},

journal = {iScience},

volume = {26},

issue = {3},

pages = {106165},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{hc22:biorxiv,

title = {Engineering stringent genetic biocontainment of yeast with a protein stability switch},

author = {S. A. Hoffmann and Y. Cai},

doi = {10.1101/2022.11.24.517818 },

year  = {2022},

date = {2022-11-24},

journal = {bioRxiv},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{jlyzc22:biorxiv,

title = {Reconstruct a eukaryotic chromosome arm by de novo design and synthesis},

author = {S. Jiang and Z. Luo and K. Yu and S. Zhao and Z. Cai and W. Yu and H. Wang and L. Cheng and Z. Liang and H. Gao and M. Monti and D. Schindler and L. Huang and C. Zeng and W. Zhang and C. Zhou and Y. Tang and T. Li and Y. Ma and Y. Cai and J. D. Boeke and J. Dai},

doi = {10.1101/2022.10.04.509869 },

year  = {2022},

date = {2022-10-04},

journal = {bioRxiv},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{swjbw22:biorxiv,

title = {Design, Construction, and Functional Characterization of a tRNA Neochromosome in Yeast},

author = {D. Schindler and R. S. K. Walker and S. Jiang and A. N. Brooks and Y. Wang and C. A. Müller and C. Cockram and Y. Luo and A. García and D. Schraivogel and J. Mozziconacci and B. A. Blount and J. Cai and L. Ogunlana and W. Liu and K. Jönsson and D. Abramczyk and E. Garcia-Ruiz and T. W. Turowski and R. Swidah and T. Ellis and F. Antequera and Y. Shen and C. A. Nieduszynski and R. Koszul and J. Dai and L. M. Steinmetz and J. D. Boeke and Y. Cai},

doi = {10.1101/2022.10.03.510608 },

year  = {2022},

date = {2022-10-03},

journal = {bioRxiv},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{sgwwz22:biorxiv,

title = {Dissecting aneuploidy phenotypes by constructing Sc2.0 chromosome VII and SCRaMbLEing synthetic disomic yeast},

author = {Y. Shen and F. Gao and Y. Wang and Y. Wang and J. Zheng and J. Gong and J. Zhang and Z. Luo and D. Schindler and Y. Deng and W. Ding and T. Lin and R. Swidah and H. Zhao and S. Jiang and C. Zeng and S. Chen and T. Chen and Y. Wang and Y. Luo and L. Mitchell and J. S. Bader and G. Zhang and X. Shen and J. Wang and X. Fu and J. Dai and J. D. Boeke and H. Yang and X. Xu and Y. Cai},

doi = {10.1101/2022.09.01.506252},

year  = {2022},

date = {2022-09-01},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{wkxwl22:biorxiv,

title = {Laboratory evolution and polyploid SCRaMbLE reveal genomic plasticity to synthetic chromosome defects and rearrangements},

author = {T. C. Williams and H. Kroukamp and X. Xu and E. L. I. Wightman and B. Llorente and A. R. Borneman and A. C. Carpenter and N. V. Wyk and M. I. Espinosa and E. L. Daniel and R. S. K. Walker and Y. Cai and H. K. M. Nevalainen and N. C. Curach and I. W. Deveson and T. R. Mercer and D. L. Johnson and L. A. Mitchell and J. S. Bader and G. Stracquadanio and J. D. Boeke and H. D. Goold and I. S. Pretorius and I. T. Paulsen},

doi = {10.1101/2022.07.22.501046 },

year  = {2022},

date = {2022-07-22},

journal = {bioRxiv},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{bldjs22:biorxiv,

title = {Synthetic yeast chromosome XI design enables extrachromosomal circular DNA formation on demand},

author = {B. A. Blount and X. Lu and M. R. M. Driessen and D. Jovicevic and M. I. Sanchez and K. Ciurkot and Y. Zhao and S. Lauer and R. M. McKiernan and G.-O. F. Gowers and F. Sweeney and V. Fanfani and E. Lobzaev and K. Palacios-Flores and R. Walker and A. Hesketh and S. G. Oliver and Y. Cai and G. Stracquadanio and L. A. Mitchell and J. S. Bader and J. D. Boeke and T. Ellis},

doi = {10.1101/2022.07.15.500197 },

year  = {2022},

date = {2022-07-15},

journal = {bioRxiv},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zchly22:biorxiv,

title = {Debugging and consolidating multiple synthetic chromosomes reveals combinatorial genetic interactions},

author = {Y. Zhao and C. Coelho and A. L. Hughes and L. Lazar-Stefanita and S. Yang and A. N. Brooks and R. S. K. Walker and W. Zhang and S. Lauer and C. Hernandez and L. A. Mitchell and N. Agmon and Y. Shen and J. Sall and V. Fanfani and A. Jalan and J. Rivera and F.-X. Liang and G. Stracquadanio and L. M. Steinmetz and Y. Cai and J. D. Boeke},

doi = {10.1101/2022.04.11.486913},

year  = {2022},

date = {2022-04-11},

urldate = {2022-04-11},

journal = {bioRxiv},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{shgc22:co_biotech,

title = {New Opportunities for Genetic Code Expansion in Synthetic Yeast},

author = {J. Sanders and S. A. Hoffmann and A. P. Green and Y. Cai},

year  = {2022},

date = {2022-01-01},

journal = {Current Opinion in Biotechnology},

volume = {75},

number = {102691},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{jjmlf22:acs_synthbio,

title = {Automation and Expansion of EMMA Assembly for Fast-Tracking Mammalian System Engineering},

author = {J. S. James and S. Jones and A. Martella and Y. Luo and D. I. Fisher and Y. Cai},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {ACS Synthetic Biology},

volume = {11},

number = {2},

pages = {587--595},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ljjmc22:acs_synthbio,

title = {EMMA-CAD: Design Automation for Synthetic Mammalian Constructs},

author = {Y. Luo and J. S. James and S. Jones and A. Martella and Y. Cai},

year  = {2022},

date = {2022-01-01},

urldate = {2022-01-01},

journal = {ACS Synthetic Biology},

volume = {11},

number = {2},

pages = {579--586},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{osmsd21:bio_basel,

title = {SCRaMbLE: A Study of Its Robustness and Challenges through Enhancement of Hygromycin B Resistance in a Semi-Synthetic Yeast},

author = {J. Y. Ong and R. Swidah and M. Monti and D. Schindler and J. Dai and Y. Cai},

doi = {10.3390/bioengineering8030042},

year  = {2021},

date = {2021-01-01},

journal = {Bioengineering (Basel)},

volume = {8},

number = {3},

pages = {42},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{lyxms21:gb,

title = {Compacting a synthetic yeast chromosome arm},

author = {Z. Luo and K. Yu and S. Xie and M. Monti and D. Schindler and Y. Fang and S. Zhao and Z. Liang and S. Jiang and M. Luan and C. Xiao and Y. Cai and J. Dai},

year  = {2021},

date = {2021-01-01},

journal = {Genome Biology},

volume = {22},

number = {5},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{torres-garcia2020a,

title = {SpEDIT: A fast and efficient CRISPR/Cas9 method for fission yeast},

author = {S. Torres-Garcia and L. Pompeo and L. Eivers and B. Gaborieau and S. A. White and A. L. Pidoux and P. Kanigowska and I. Yaseen and Y. Cai and R. C. Allshire},

doi = {10.12688/wellcomeopenres.16405.1},

year  = {2020},

date = {2020-01-01},

journal = {Wellcome open research},

volume = {5},

pages = {274},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{dbljc20:gb,

title = {Sc3.0: revamping and minimizing the yeast genome},

author = {J. Dai and J. D. Boeke and Z. Luo and S. Jiang and Y. Cai},

year  = {2020},

date = {2020-01-01},

journal = {Genome Biology},

volume = {21},

number = {205},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{lhjcd20:ecr,

title = {Probing eukaryotic genome functions with synthetic chromosomes},

author = {Z. Luo and S. A. Hoffmann and S. Jiang and Y. Cai and J. Dai},

doi = {10.1016/j.yexcr.2020.111936},

year  = {2020},

date = {2020-01-01},

journal = {Experimental Cell Research},

volume = {390},

number = {1},

pages = {111936},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mhask20:nature_biot,

title = {The COVID-19 XPRIZE and the need for scalable, fast, and widespread testing},

author = {M. J. MacKay and A. C. Hooker and E. Afshinnekoo and M. Salit and J. Kelly and J. V. Feldstein and N. Haft and D. Schenkel and S. Nambi and Y. Cai and F. Zhang and G. Church and J. Dai and C. L. Wang and S. Levy and J. Huber and H. P. Ji and A. Kriegel and A. L. Wyllie and C. E. Mason},

year  = {2020},

date = {2020-01-01},

journal = {Nature Biotechnology},

volume = {38},

pages = {1021--1024},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zypgm20:acs_synthbio,

title = {Leveraging the Hermes Transposon to Accelerate the Development of Nonconventional Yeast-based Microbial Cell Factories},

author = {Y. Zhao and Z. Yao and D. Ploessl and S. Ghosh and M. Monti and D. Schindler and M. Gao and Y. Cai and C. Yang and M. Qiao and M. Cao and Z. Shao},

doi = {10.1021/acssynbio.0c00123},

year  = {2020},

date = {2020-01-01},

urldate = {2020-01-01},

journal = {ACS Synthetic Biology},

volume = {9},

number = {7},

pages = {1736--1752},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{zgjlj19:biochem,

title = {Multiplex Genome Engineering for Optimizing Bioproduction in Saccharomyces cerevisiae},

author = {J. Y. Auxillos and E. Garcia-Ruiz and S. Jones and T. Li and S. Jiang and J. Dai and Y. Cai},

year  = {2019},

date = {2019-01-01},

journal = {Biochemistry},

volume = {58},

number = {11},

pages = {1492--1500},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{hcccc19:nature_comm,

title = {Building a global alliance of biofoundries},

author = {N. Hillson and M. Caddick and Y. Cai and J. A. Carrasco and M. W. Chang and N. C. Curach and D. J. Bell and R. Le Feuvre and D. C. Friedman and X. Fu and N. D. Gold and M. J. Herrgard and M. B. Holowko and J. R.Johnson and R. A. Johnson and J. D. Keasling and R. I. Kitney and A. Kondo and C. Liu and V. J. J. Martin and F. Menolascina and C. Ogino and N. J. Patron and M. Pavan and C. L. Poh and I. S. Pretorius and S. J.Rosser and N. S. Scrutton and M. Storch and H. Tekotte and E. Travnik and C. E. Vickers and W. S. Yew and H. Zhao and Y. Yuan and P. S. Freemont},

year  = {2019},

date = {2019-01-01},

urldate = {2019-01-01},

journal = {Nature Communications},

volume = {10},

pages = {2040},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{jmc19:methods_enzym,

title = {EMMA assembly explained: A step-by-step guide to assemble synthetic mammalian vectors},

author = {S. Jones and A. Martella and Y. Cai},

year  = {2019},

date = {2019-01-01},

journal = {Methods in Enzymology},

volume = {617},

pages = {463--493},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{lzzcd19:acs_synthbio,

title = {Improving Chromosome Synthesis with a Semiquantitative Phenotypic Assay and Refined Assembly Strategy},

author = {Y. Lin and X. Zou and Y. Zheng and Y. Cai and J. Dai},

year  = {2019},

date = {2019-01-01},

journal = {ACS Synthetic Biology},

volume = {8},

number = {10},

pages = {2203--2211},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{avumc18:front_bioeng_biotech,

title = {Hydrogen Peroxide-Based Fluorometric Assay for Real-Time Monitoring of SAM-Dependent Methyltransferases},

author = {M. K. Akhtar and D. Vijay and S. Umbreen and C. J. McLean and Y. Cai and D. J. Campopiano and G. J.Loake},

doi = {10.3389/fbioe.2018.00146},

year  = {2018},

date = {2018-10-01},

journal = {Frontiers in Bioengineering and Biotechnology},

volume = {6},

pages = {146},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{sdc18:co_chembio,

title = {Synthetic genomics: a new venture to dissect genome fundamentals and engineer new functions},

author = {D. Schindler and J. Dai and Y. Cai},

year  = {2018},

date = {2018-10-01},

journal = {Current Opinion in Chemical Biology},

volume = {46},

pages = {56--62},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{galdc18:methods_enzym,

title = {YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast},

author = {E. Garcia-Ruiz and J. Auxillos and T. Li and J. Dai and Y. Cai},

doi = {10.1016/bs.mie.2018.05.003},

year  = {2018},

date = {2018-01-01},

journal = {Methods in Enzymology},

volume = {608},

pages = {277--306},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{lccd18:metab_eng,

title = {Artificial Protein Scaffold System (AProSS): An efficient method to optimize exogenous metabolic pathways in Saccharomyces cerevisiae},

author = {T. Li and X. Chen and Y. Cai and J. Dai},

year  = {2018},

date = {2018-01-01},

journal = {Metabolic Engineering},

volume = {49},

pages = {13--20},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{llwpt18:nature_comm,

title = {Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods},

author = {W. Liu and Z. Luo and Y. Wang and N. T. Pham and L. Tuck and I. Pérez-Pi and L. Liu and Y. Shen and C. French and M. Auer and J. Marles-Wright and J. Dai and Y. Cai},

year  = {2018},

date = {2018-01-01},

journal = {Nature Communications},

volume = {9},

pages = {1936},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{lwwzg18:nature_comm,

title = {Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES},

author = {Z. Luo and L. Wang and Y. Wang and W. Zhang and Y. Guo and Y. Shen and L. Jiang and Q. Wu and C. Zhang and Y. Cai and J. Dai},

year  = {2018},

date = {2018-01-01},

journal = {Nature Communications},

volume = {9},

pages = {1930},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{lygjy18:scls,

title = {Whole genome engineering by synthesis},

author = {Z. Luo and Q. Yang and B. Geng and S. Jiang and S. Yang and X. Li and Y. Cai and J. Dai},

year  = {2018},

date = {2018-01-01},

journal = {Science China Life Sciences},

volume = {61},

number = {12},

pages = {1515--1527},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{atysi17:pnas,

title = {Low escape-rate genome safeguards with minimal molecular perturbation of Saccharomyces cerevisiae},

author = {N. Agmon and Z. Tang and K. Yang and B. Sutter and S. Ikushima and Y. Cai and K. Caravelli and J. A. Martin and X. Sun and W. J. Choi and A. Zhang and G. Stracquadanio and H. Hao and B. P. Tu and D. Fenyö and J. S. Bader and J. D. Boeke},

year  = {2017},

date = {2017-01-01},

urldate = {2017-01-01},

journal = {Proceedings of the National Academy of Sciences},

volume = {114},

pages = {1470-- 1479},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{cd17:csh_proto,

title = {Methods to Synthesize Large DNA Fragments for a Synthetic Yeast Genome},

author = {Y. Cai and J. Dai},

year  = {2017},

date = {2017-01-01},

journal = {Cold Spring Harbor Protocols},

volume = {2017},

number = {3},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{chdjm17:acs_synthbio,

title = {LeafLIMS: A Flexible Laboratory Information Management System with a Synthetic Biology Focus},

author = {T. Craig and R. Holland and R. D’Amore and J. R. Johnson and H. V. McCue and A. West and V. Zulkower and H. Tekotte and Y. Cai and D. Swan and R. P. Davey and C. Hertz-Fowler and A. Hall and M. Caddick},

year  = {2017},

date = {2017-01-01},

journal = {ACS Synthetic Biology},

volume = {6},

number = {12},

pages = {2273--2280},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{jqlpl17:acs_synthbio,

title = {Orthogonal Ribosome Biofirewall},

author = {B. Jia and H. Qi and B. Z. Li and S. Pan and D. Liu and H. Liu and Y. Cai and Y. J. Yuan},

year  = {2017},

date = {2017-01-01},

journal = {ACS Synthetic Biology},

volume = {6},

number = {11},

pages = {2108--2117},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{ltwc17:methods_molec_bio,

title = {Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways},

author = {W. Liu and L. R. Tuck and J. M. Wright and Y. Cai},

year  = {2017},

date = {2017-01-01},

journal = {Methods in Molecular Biology},

volume = {1642},

pages = {285--302},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mmapc17:acs_synthbio,

title = {EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors},

author = {A. Martella and M. Matjusaitis and J. Auxillos and S. M. Pollard and Y. Cai},

year  = {2017},

date = {2017-01-01},

journal = {ACS Synthetic Biology},

volume = {6},

number = {7},

pages = {1380--1392},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mmsyz17:science,

title = {3D organization of synthetic and scrambled chromosomes},

author = {G. Mercy and J. Mozziconacci and V. F. Scolari and K. Yang and G. Zhao and A. Thierry and Y. Luo and L. A. Mitchell and M. Shen and Y. Shen and R. Walker and W. Zhang and Y. Wu and Z. X. Xie and Z. Luo and Y. Cai and H. Yang and J. Dai and Y. J. Yuan and J. D. Boeke and J. S. Bader and H. Muller and R. Koszul},

year  = {2017},

date = {2017-01-01},

urldate = {2017-01-01},

journal = {Science},

volume = {355},

number = {6329},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{mwskw17:science,

title = {Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond},

author = {L. A. Mitchell and A. Wang and G. Stracquadanio and Z. Kuang and X. Wang and K. Yang and J. A. Martin and S.Richardson and Y. Zhao and R. Walker and Y. Luo and H. Dai and K. Dong and Z. Tang and Y. Cai and Y. Yang and A. Heguy and B. Ueberheide and D. Fenyö and J. Dai and J. S. Bader and J. D. Boeke},

year  = {2017},

date = {2017-01-01},

urldate = {2017-01-01},

journal = {Science},

volume = {355},

number = {6329},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{rmsyd17:science,

title = {Design of a synthetic yeast genome},

author = {S. M. Richardson and L. A. Mitchell and G. Stracquadanio and K. Yang and J. S. Dymond and J. E. DiCarlo and D. Lee and C. L. Huang and S. Chandrasegaran and Y. Cai and J. D. Boeke and J. S. Bader},

year  = {2017},

date = {2017-01-01},

urldate = {2017-01-01},

journal = {Science},

volume = {355},

number = {6329},

pages = {1040--1044},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{swcgg17:science,

title = {Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome},

author = {Y. Shen and Y. Wang and T. Chen and F. Gao and J. Gong and D. Abramczyk and R. Walker and H. Zhao and W. Liu and S. Chen and Y. Luo and C. A. Müller and A. Paul-Dubois-Taine and B. Alver and G. Stracquadanio and L. A. Mitchell and Z. Luo and Y. Fan and B. Zhou and B. Wen and F. Tan and Y. Wang and J. Zi and Z. Xie and B. Li and K. Yang and S. M. Richardson and H. Jiang and C. E. French and C. A. Nieduszynski and R. Koszul and A. L.Marston and Y. Yuan and J. Wang and J. S. Bader and J. Dai and J. D. Boeke and X. Xu and Y. Cai and H. Yang},

year  = {2017},

date = {2017-01-01},

urldate = {2017-01-01},

journal = {Science},

volume = {355},

number = {6329},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{wlzmx17:science,

title = {Bug mapping and fitness testing of chemically synthesized chromosome X},

author = {Y. Wu and B. Z. Li and M. Zhao and L. A. Mitchell and Z. X. Xie and Q. H. Lin and X. Wang and W. H. Xiao and Y. Wang and X. Zhou and H. Liu and X. Li and M. Z. Ding and D. Liu and L. Zhang and B. L. Liu and X. L. Wu and F. F. Li and X. T. Dong and B. Jia and W. Z. Zhang and G. Z. Jiang and Y. Liu and X. Bai and T. Q. Song and S. J. Zhou and Y. Chen and R. Y. Zhu and F. Gao and Z. Kuang and X. Wang and M. Shen and K. Yang and S. M. Richardson and G. Stracquadanio and Y. Lin and L. Wang and R. Walker and Y. Luo and P. S. Ma and Y. Cai and H. Yang and J. Dai and J. S. Bader and J. D. Boeke and Y. J. Yuan},

year  = {2017},

date = {2017-01-01},

urldate = {2017-01-01},

journal = {Science},

volume = {353},

number = {6329},

keywords = {},

pubstate = {published},

tppubtype = {article}

}

@article{xlmwq17:science,

title = {"Perfect" designer chromosome V and behavior of a ring derivative},

author = {Z. X. Xie and B. Z. Li and L. A. Mitchell and Y. Wu and X. Qi and Z. Jin and B. Jia and X. Wang and B. X. Zeng and H. M. Liu and X. L. Wu and Q. Feng and W. Z. Zhang and W. Liu and M. Z. Ding and X. Li and G. R. Zhao and J. J. Qiao and J. S. Cheng and M. Zhao and Z. Kuang and X. Wang and J. A. Martin and G. Stracquadanio and K. Yang and X. Bai and J. Zhao and M. L. Hu and Q. H. Lin and W. Q. Zhang and M. H. Shen and S. Chen and W. Su and E. X. Wang and R. Guo and F. Zhai and X. J. Guo and H. X. Du and J. Q. Zhu and T. Q. Song and J. J. Dai and F. F. Li and G. Z. Jiang and S. L. Han and S. Y. Liu and Z. C. Yu and X. N. Yang and K. Chen and C. Hu and D. S. Li and N. Jia and Y. Liu and L. T. Wang and S. Wang and X. T. Wei and M. Q. Fu and L. M. Qu and S. Y. Xin and T. Liu and K. R. Tian and X. N. Li and J. H. Zhang and L. X. Song and J. G. Liu and J. F. Lv and H. Xu and R. Tao and Y. Wang and T. T. Zhang and Y. X. Deng and Y. R. Wang and T. Li and G. X. Ye and X. R. Xu and Z. B. Xia and W. Zhang and S. L. Yang and Y. L. Liu and W. Q. Ding and Z. N. Liu and J. Q. Zhu and N. Z. Liu and R. Walker and Y. Luo and Y. Wang and Y. Shen and H. Yang and Y. Cai and P. S. Ma and C. T. Zhang and J. S. Bader and J. D. Boeke and Y. J. Yuan},

year  = {2017},

date = {2017-01-01},

journal = {Science},

volume = {355},

number = {6329},

keywords = {},

pubstate = {published},

tppubtype = {article}

}